A5231
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 | Immunoblotting Tissue extracts and cell lysates were separated on SDS-PAGE and blotted with Anti-Abi1 (Cat. No. A5231). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution 1:3,000. Lanes 1,6. rat brain 2. mouse brain 3. A431 4. HEK293T cells transfected with human Abi1 5. HEK293T cells untransfected 6. Inhibition with Abi1 immunizing peptide (human, 491-508) (10 μg/mL) |
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A5231
Sigma
Anti-Abi1 (C-terminal) antibody produced in rabbit
~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
| Synonym: | Anti-Abelson interactor 1, Anti-Abl-interactor protein 1, Anti-eps8 binding protein, Anti-Interactor protein AblBP4, Anti-Nap1 binding protein NAP1BP, Anti-spectrin SH3 domain binding protein 1 SSH3BP1 |
Description
| Immunogen | synthetic peptide corresponding to amino acids 492-508 located at the C-terminus of human Abi1, conjugated to KLH. This sequence is identical in several species including mouse, rat, and dog Abi1. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | affinity isolated antibody |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~65 kDa |
| species reactivity | human, rat |
| concentration | ~1.5 mg/mL |
| application(s) | immunoblotting: 0.5-1.0 μg/mL using rat brain cytosolic fraction (S1) |
| immunoblotting: 1-2 μg/mL using A431 cell lysate | |
| Gene Information | human ... ABI1(10006) |
| rat ... Abi1(79249) | |
| mouse ... Abi1(11308) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
Related Products
| Related product | A5106, Anti-ABI1 antibody produced in rabbit |
